IGF-I binding in primary culture of muscle cells of rainbow trout: changes during in vitro development.

IGF-I binding in primary culture of muscle cells of rainbow trout (Oncorhynchus mykiss): changes during in vitro development

Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout.

The relative function of IGF-I and insulin on fish muscle metabolism and growth has been investigated by the isolation and culture at different stages (myoblasts at day 1, myocytes at day 4, and myotubes at day 10) of rainbow trout muscle cells. This in vitro model avoids interactions with endogenous peptides, which could interfere with the muscle response. In these cells, the effects of IGF-I ...

Expression of rainbow trout glucose transporters GLUT1 and GLUT4 during in vitro muscle cell differentiation and regulation by insulin and IGF-I.

Insulin is an important factor for the maintenance of glucose homeostasis, enhancing glucose uptake in its target tissues in a process that has been conserved between fish and mammals. In fish skeletal muscle cells, like in mammals, insulin promotes GLUT4 translocation to the plasma membrane and, consequently, glucose uptake, but its role regulating the expression of glucose transporters in vit...

Role of insulin and IGFs in fish muscle development and quality

In order to identify possible relationships between insulin, Insulin-like Growth Factors (IGFs) and the various transcription factors involved in cell proliferation and muscle growth (such as MyoD, myogenin, myostatin), we combined in vivo and in vitro experiments. Changes in food regimen in rainbow trout (Onchorhynchus mykiss) altered the IGF system at peptide and receptor level. The expressio...

IGF-I and insulin receptor signal transduction in trout muscle cells.

In this study, primary cultures of trout skeletal muscle cells were used to investigate the main signal transduction pathways of insulin and IGF-I receptors in rainbow trout muscle. At different stages of in vitro development (myoblasts on day 1, myocytes on day 4, and fully developed myotubes on day 11), we detected in these cells the presence of immunoreactivity against ERK 1/2 MAPK and Akt/P...